Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Early Detection of Aortic Degeneration in a Mouse Model of Sporadic Aortic Aneurysm and Dissection Using Nanoparticle Contrast-Enhanced Computed Tomography

doi: 10.1161/atvbaha.120.315210

Figure Lengend Snippet: Figure 6. Intramural accumulation of nanoparticle computed tomography (CT) contrast agent associated with endothelial cell injury and increased endothelial permeability. A, Immunofluorescence staining shows the expression of necroptosis cell death marker RIP3 (receptor-interacting protein kinase 3) in aortic endothelial cells (ECs) of challenged mice. In comparison, aortic ECs of unchallenged control mice do not show expression of RIP3. Color code: RIP3, green; PECAM1 (platelet endothelial cell adhesion molecule 1) purple; nanoparticle, red; nuclei 4′,6-diamidino-2-phenylindole (DAPI; blue). B, Aortas of challenged mice show significantly higher uptake of Evans blue (EB) dye than do aortas of unchallenged control mice. Analysis was performed for ascending aortic segments. n=5 mice/group. P<0.05 indicates statistical significance. C, Representative gross microscopic image of excised aorta and ex vivo pseudocolored CT image of a challenged mouse and a control mouse that underwent EB dye injection. Note the presence of pathology in the ascending segment (red arrow) in the gross image and the intramural CT signal (white arrow) in the ex vivo CT image of the challenged mouse. Ang II indicates angiotensin II; HFD, high-fat diet; and WT, wild type.

Article Snippet: DOI: 10.1161/ATVBAHA.120.315210 April 2021 1537 were stained with anti-RIP3 antibody (Cell Signaling, Danvers, MA; 95702S, 2 μg/mL) and double stained with anti-PECAM1 (platelet endothelial cell adhesion molecule 1; CD31) antibody (Abcam, Cambridge, MA; ab24590, 2 μg/mL) for endothelial cells.

Techniques: Computed Tomography, Permeability, Immunofluorescence, Staining, Expressing, Marker, Comparison, Control, Ex Vivo, Injection